In-Vitro Cytotoxic and Apoptotic Effect of Wrightia tinctoria Roxb. Alcoholic Extract/Fractions

Charanjeet H Singh, Dharmendra Ahuja, S C Mehta

Abstract


The ayurvedic medicine system of prescription suggests Wrightia tinctoria Roxb. (Apocynaceae), for the treatment of tumors. The investigation is required as malignant growth is the second most basic reason for death on the planet; the greater part of the customary medications have appeared, minimal effort approaches, and higher harmfulness of the current drugs. The present examination means to decide the method of cell death instigated by the alcoholic extract fractions of Wrightia tinctoria on human malignant growth cell lines. Amongst crude alcoholic extract of Wrightia tinctoria Roxb. investigate for potential anticancer action, there are two fractions selected for investigation these are WTD (dichloromethane fraction) and WTE (ethyl acetate fraction) hey were found to have capable cytotoxic potential on all cancerous cell lines contemplated by MTT test and SRB test. Ethyl acetate fraction found to have increasingly powerful action in A549, U343HeLa, BT549 and HCT15 cells lines. Strangely, ethyl acetate was less cytotoxic in HBL-100 cell line, demonstrate the particular action towards malignant growth cells. AO/EB staining assay and Hoechst-33342 staining assay demonstrate membrane blebbing, consolidated and fragmented nuclei upon treatment with ethyl acetate and dichloromethane fraction in A549, U343 and HeLa cell lines. DNA fragmentation stepping stool and genomic DNA discontinuity were seen with DNA fragmentation test dependent on gel electrophoresis and COMET test by fluorescent microscopy method. Essentially, cell cycle examination by flow cytometer demonstrates mutilation of ordinary cell cycle and expanded subG0phase. Ethyl acetate fraction was found genotoxic by micronuclei development. These outcomes show that both fractions instigate apoptosis however not cells decomposition in the malignant growth cells. This plant keeps potent anticancer activity in vivo and further researches are to recognize the responsible phytoconstituents and mechanism of action.

Keywords


MTT, SRB, Wrightia tinctoria Roxb, Cytotoxicity, Apoptosis.

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DOI: http://dx.doi.org/10.25258/ijpqa.10.2.25

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