A simple, highly sensitive, isocratic stability indicating reversed phase-high performance liquid chromatography (RP-HPLC) method with UV detection at 247 nm was developed and validated for analysis of Atorvastatin (AT). Diclofenac was used as an internal standard (IS). Retention times of the AT and diclofenac (IS) were found to be 4.2 and 8.2 min, respectively. A mobile phase consisting of 0.05 M sodium phosphate buffer and methanol (30:70 v/v) pH 4.1 at flow rate 1ml/min was employed in this study. The calibration curves were linear with regression coefficient (r2 detection (LOD) and the limits of quantitation (LOQ) were found out to be 5.0 and 15 ng/ml, respectively. The method was statistically validated in accordance with International Conference on Harmonization (ICH) guidelines. The proposed method is sensitive, specific and was successfully applied for the estimation of atorvastatin in pharmaceutical formulations (bulk drug, tablet, nanoemulsion) and in rat serum pharmacokinetic studies.

This communication presents an analysis of physico-chemical characteristics of groundwater quality of Piro and Jagdishpur blocks of Bhojpur District of Bihar. Total 40 numbers of groundwater samples were collected from different locations of these blocks and analysed for the parameters pH, electrical conductivity (EC), total dissolved solid(TDS), total hardness(TH),  Calcium(Ca2+), magnesium(Mg2+),fluoride(F-), iron(Fe), sulphate(SO42-)and arsenic (As). Water samples were collected from shallow hand pumps, deep hand pumps and public water supply.