Rasa is important group of formulation used by Ayurvedic Physicians to treat various types of diseases. Krimimudgara Rasa, as per Ayurvedic literatures is used as an anthelmintic drug. It expels the worms present in cough and faeces of human and gives relief from symptoms like indigestion, vomiting, fever, sneezing and pain in stomach. Many manufactures are adding adulterants in Ayurvedic formulations, which render them less effective and lack of phyto/biomarker(s) responsible for their therapeutic efficacy. No method has been developed to check quantity of phytomarker (s) so far. In the present study an attempt has been made to develop a HPTLC method of quantitative estimation of marker compounds, embelin and strychnine in laboratory prepared formulation (LF) and a marketed formulation (MF) of Krimimudgara Rasa. The laboratory formulation (LF) was found to contain 0.254 % of embelin and 0.080 % of strychnine while the market formulation (MF) shows 0.135 % of embelin and 0.046 % of strychnine. The method develop was found to be accurate, precise, reproducible & simple for quantitative estimation of phytomarkers i.e. embelin and strychnine and can be recommended for routine analysis of formulation containing such drugs.