Rasa is important group of formulation used by Ayurvedic Physicians to treat various types of diseases. Krimimudgara Rasa, as per Ayurvedic literatures is used as an anthelmintic drug. It expels the worms present in cough and faeces of human and gives relief from symptoms like indigestion, vomiting, fever, sneezing and pain in stomach. Many manufactures are adding adulterants in Ayurvedic formulations, which render them less effective and lack of phyto/biomarker(s) responsible for their therapeutic efficacy. No method has been developed to check quantity of phytomarker (s) so far. In the present study an attempt has been made to develop a HPTLC method of quantitative estimation of marker compounds,  embelin  and  strychnine  in  laboratory  prepared  formulation  (LF)  and  a  marketed  formulation  (MF)  of Krimimudgara Rasa. The laboratory formulation (LF) was found to contain 0.254 % of embelin and 0.080 % of strychnine while the market formulation (MF) shows 0.135 % of embelin and 0.046 % of strychnine. The method develop was found to be accurate, precise, reproducible & simple for quantitative estimation of phytomarkers i.e. embelin and strychnine and can be recommended for routine analysis of formulation containing such drugs.

A simple, rapid and much economic spectrophotometric method in ultraviolet region has been developed for determination of aceclofenac in bulk and in pharmaceutical formulations. Aceclofenac has its absorption maxima at 273.2 nm in phosphate buffer. The linearity was observed in the concentration range 5-40 mcg/ml with correlation coefficient 0.9992. The same method was applied for the estimation of drug in a tablet dosage form and found to be feasible and reproducible. The method is found to be compatible with dissolution media as the UV cutoff for phosphate buffer is <200 nm. The proposed method was validated statistically as per ICH guidelines and found to be accurate and reproducible for the routine dissolution testing of aceclofenac in pharmaceutical dosage form.

A simple, rapid, accurate and precise assay procedure based on Spectrophotometric method has been developed for the estimation of Escitalopram oxalate (ESC) in Pharmaceutical formulation. The method was based on the bromination of ESC with a known excess amount of bromate-bromide mixture in acid medium followed by the determination of surplus bromine by reacting with dye methyl orange and measuring the absorbance at 507 nm. The proposed method was linear over the range of 2-14 μg/mL with the correlation co-efficient (r) of 0.9983 and the mean recovery for ESC was 100.5 %. The intermediate precision data obtained under different experimental setup, the calculated value of co-efficient of variance (CV, %) was found to be 1.14 % for both day to day and within a day variation. The proposed method can be successfully applied for the analysis of tablet formulations.

Losartan, is highly effective blood pressure lowering agent, which directly works by selectively blocking angiotensin-I (AT-I) receptor. It is primarily metabolized in active form, Losartan carboxylic acid (LCA). A sensitive and selective LC-MS/MS method for the determination of Losartan and Losartan Carboxylic Acid in human plasma was developed and validated. The ammonium formate: methanol (50:50 V/V) buffer was selected as the mobile phase; as the retention time was about only 1 minute and valsartan was taken as internal standard, due to resemble structural and chemical nature with the Losartan.  The results were reproducible and precise with the samples prepared by solid phase extraction technique using “OASIS” HLB Cartridges (30mg), during method development trials; therefore it was used in sample preparation, for whole bioanalytical study of Losartan. The limit of quantification was from 25 ng /ml to 1500 ng/ml.

UPLC  can  be  regarded  as  a  new  direction  for  liquid  chromatography. UPLC  refers  to  Ultra  Performance  Liquid Chromatography.UPLC, improve in three areas: ‘‘speed, resolution, and sensitivity’’. In this system uses fine particles (less than 2.5µm) so decreases the lengh of column, saves time and reduces solvent consumption.