A simple, accurate, low cost and specific HPTLC method for estimation of Fluoxetine hydrochloride in capsule has been developed. It was performed on Silica gel G60 F254 aluminium foil using Acetone : Methanol in the ratio of 5:4 as mobile phase. The mobile phase containing chamber was saturated for 10 minutes at room temperature. The Rf value of Fluoxetine was found to be 0.12. The plate was scanned and quantified at 226 nm. The calibration curve response was observed between 300-2100 ng. The linear regression data showed good linear relationship of r2 = 0.999. The percent recovery was found to be 99.94 ±1.188. The developed method was validated for its accuracy and precision with suitable parameters.

The   present   communication   attempts   to   investigate   pharmacognostical   and   physicochemical   analysis   and chromatographic profiles of Butea monosperma (Lam.) Kuntze (fabaceae). Flowers are used as drug in many ailments like eye disease, chronic fever, enlargement of spleen, leucorrhoea, epilepsy, leprosy and gout etc. Identification of plants with botanical verifications is essential as adulteration due to misidentification of plant species or parts are common.  Standardization of medicinal plant product is the prime need of the current time. The significant popularity of HPTLC in the analytical testing of pharmaceutical, bulk drugs and herbal lends its fame to the attributes. Therefore, HPTLC studies of alcoholic extract of Butea monosperma flowers were also carried out to lay down the fingerprint profile of drug. The study revealed specific identities for Butea monosperma (Lam.) Kuntze, which may play a key role in identification of plant and can be useful in standardization of the herbal drugs.

A simple and cost effective spectrophotometric method is described for the determination of Torsemidein pure form and in pharmaceutical formulations. The method is based on the formation derivative when the drug reacts with picric acid solution. The colored derivative solution has an absorption maximum at 350 nm; also obeys Beer’s law in the concentration range 5-25μg /mL. The absorbance was found to increase linearly with increasing concentration of torsemide, which is corroborated by the calculated correlation coefficient value of 0.997. The slope and intercept of the equation of the regression line are 0.035 and 0.002 respectively. The optimum experimental parameters for the reaction have been studied. The validity of the described procedure was assessed. Statistical analysis of the results has been carried out revealing high accuracy and good precision. The proposed method was successfully applied to the determination of Torsemide in pharmaceutical formulations.

In the present study, an attempt has been made to develop an analytical method for the simultaneous estimation of curcuminoids and gallic acid in commercially marketed ayurvedic polyherbal formulation Nisha Amalaki vatti by spectrophotometric   method. Simultaneous   equations   (Vierodt’s   method)   were   performed   by   UV/Visible spectrophotometric. Curcuminoids has absorbance maxima at about 427nm and Gallic acid maxima at about 227nm in methanol. The linearity was obtained in the concentration range of 10-50 mcg/mL for both curcuminoids and gallic acid. The results were of the analysis were validated statistically and the recovery studies were carried out as per ICH guidelines.

A simple, sensitive, rapid and reproducible reversed- phase HPLC method has been developed and validated for estimation of Olmesartan medoxomil and Hydrochlorothiazide simultaneously and also the comparative study of invitro data in tablet formulation. The assay involved an isocratic elution of these two component on Inertsil-phenyl column (25cm X 4.6mm, 5μm) using a mobile phase composition of Buffer: Acetonitirile (480:520) and pH adjusted to 3.0 with dilute orthophosphoric acid. The flow rate was 1.0 mL/min and the analytes monitored at 257nm.Calibration curves were linear with coefficient correlation between 0.99 to 1.0 over a concentration range of 24 to 74 μg/mL of Olmesartan medoxomil and 15 to 47 μg/mL for Hydrochlorothiazide respectively. The invitro release of various test units was compared for their similarity using the f2 test which limits were found with in the acceptance criteria.  All the validation parameters were with in the acceptance range according to ICH norms. The described method was successfully employed for quality control assay of both the component simultaneously and dissolution data helpful in generating the further information regarding invivo absorption rate in tablet dosage form.

A simple and sensitive spectrophotometric method has been developed for the determination of Thorium (IV) using newly synthesized reagent   3,5-Dimethoxy-4-hydroxybenzaldehyde isonicotinoyl hydrazone (DMHBIH).   Thorium (IV)  forms  an  Yellow  coloured  water  soluble  complex  with  the  reagent  in  acidic  medium  pH  3.0.  The  molar absorptivity and Sandell’s sensitivity of coloured species are 4.84 x 104 L.mol-1 cm-1 and 0.0083 μg/cm2  respectively. Beer’s law is obeyed in the range 0.580-5.80 μg/ml of Thorium(IV) at λmax  at 390 nm. Thorium (IV) forms a 1:1 complex and stability constant of the complex is 4.176 x 106.  the developed derivative spectrophotometric method has been satisfactorily applied for the determination of Thorium (IV) in  monazite sand.